I am writing to discuss the use of rat-sourced primary antibody on rat tissue.
One concern (from Andrea Hooper's last email to me) is that the 10mg/ml IgG in normal blood will result in great background, or false-positive staining.
How do you think?
My personal opinions are:
(1) perfuse the rat well.
(2) use a control group without 1st antibody, just add goat-anti-Rat IgG, for example (care about the cross reactivity)
(3) use DAPI staining to see if the staings are located in cell, rather by blood contamination.
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