In the June 2008 issue of CAP Today, I ran across a fixative additive
called PhosphoGuard from Targeted Molecular Technologies. 

They claim it prevents degradation of phosphorylated and activated
proteins allowing for better IHC detection (stronger/more abundant
signal) by better inactivation of phosphatases compared to plain 10%
NBF.  Apparently you just add some to your formalin. 

You can check it out further on their website.
http://www.tmdlab.com/technologies_PHOSPHO.htm

Just wondering if any of you have tried it?

Brett

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_connolly@merck.com

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