In the June 2008 issue of CAP Today, I ran across a fixative additive
called PhosphoGuard from Targeted Molecular Technologies.
They claim it prevents degradation of phosphorylated and activated
proteins allowing for better IHC detection (stronger/more abundant
signal) by better inactivation of phosphatases compared to plain 10%
NBF. Apparently you just add some to your formalin.
You can check it out further on their website.
Just wondering if any of you have tried it?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
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