Quick question for everyone. We received a cytology slide in xylene
( left overnight) for Fite stain. Is this ruined because of the
extended time in xylene? I looked in the Carson book and here is
what is written
M leprae and Nocardia spp. are weakly acid fast and not alcohol fast
(Chandler) so alcohol must be avoided. So we don't want to receive it
in Alcohol
Why a short exposure to xylene for removal of paraffin has such an
adverse effect on the leprosy organism while the prolonged exposure
to xylene during processing does not have the same effect to xylene
during processing does not have the same effect is an interesting
consideration (Stevens). According to Stevens, once the leprosy
organism is adequately stained, it will resist decolonization as
tenaciously as the tubercle bacillus does; instead the problem may be
one of resistance to uptake of the stain rather than retention.
We are having quite the discussion what is right. So I need more
opinions from out there
Thanks,
Jeannette Mitchell, BS, HTL(ASCP), QIHC
R&D Histotechnologist
EP1-119
Fletcher Allen Health Care
Burlington, VT 05401
Phone # 802-847-3096
&
802-847-5362
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|