I am trying an antibody called Alexa Fluor 488 anti-mouse CD4 on fresh frozen mouse tissue.  Having only worked with AED/DAB staining, not very familiar with this. 
Does anyone have any experience with this and if you could send me some tips/protocols.  Thanks ahead of time
 
 
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology
 
(585) 275-7210
 
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