AW: [Histonet] borrelia burgdorferi protocol

From:"Gudrun Lang"

In spite of your "negative experiences" would you please share your protocol
with me?
And what do you think is the cause, why it doesn't work on human tissue?

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: Richard Cartun [] 
Gesendet: Donnerstag, 17. Juli 2008 21:00
Betreff: Re: [Histonet] borrelia burgdorferi protocol

I have been doing IHC for Borrelia burgdorferi for years and have never seen
a positive human case; only in animal tissue.


Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Gudrun Lang"  07/17/08 12:33 PM >>>
Hi all!

Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on
the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the
datasheet is no hint about pretreatment or titer. So some information would
be helpful to start with.


Thanks in advance

Gudrun Lang

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