Hello Jennifer,

I've worked in an eye institute before and i have done
a lot of frozen mouse and rat eyes. Normally i'll have
a lot of difficulties in cutting the lens as when it
is fixed in 4% PFA, it hardens.
I've read in one of the previous email from histonet
that Perenyi's fluid is a good fixative for eye as
both Nitric and chromic acids have a softening effect
on the tissuesand ethanol is a coagulant fixative. It
is good as it fixes retina really well with little
separation of the layers and does not harden the lens.
 
I hope this will help.

Jaimie

 
--- "Harvey, Jennifer Lynn"
 wrote:

> Histonet:
> 
>  
> 
> I thought years of bone work I had done some of the
> most challenging
> histology. We move to Nashville and I took a job in
> eye research. Was I
> crazy??? This is worse. I need to cut frozen
> sections on 4%PFA fixed
> monkey eyes?  Any ideas?? 
> 
>  
> 
>  
> 
> Thanks!!!!
> 
>  
> 
>  
> 
> Jennifer Harvey
> 
> Vanderbilt Eye Institute
> 
> RM 8105 MCE North Tower
> 
> 1215 21st Ave. South
> 
> Nashville, TN  37232-8808
> 
> Phone: 615-936-1486
> 
>  
> 
>  
> 
>  
> 
>  
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



      ___________________________________________________________ 
Yahoo! Mail is the world's favourite email. Don't settle for less, sign up for
your free account today http://uk.rd.yahoo.com/evt=44106/*http://uk.docs.yahoo.com/mail/winter07.html 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet