Most likely it's the Permount (maybe the xylenes). Use an aqueous
mount like glycerol, methyl salicylate*, Aquamount, Fluormount, etc.
There are others that I don't recall off hand.
After mounting, keep the slides flat. Seal with VALAP.
*Methyl salicylate is an excellent clearing medium and mountant, go
into it using your xylenes schedule. We have specimens that have been
in Me-sal for 14+ years and are still nice and bright.
Rhodamine-Phalloidin stain, not slide mounted but kept in e.g.
microfuge tubes. These aren't brain sections, but it should work for
>I am carrying out c-fos staining on brain tissue, and have run into a bit
>of a problem. The staining seems to work fine up untill I mount the sample
>with a xylene based mountant (permount). The stains are there after alcohol
>washing, but once I mount it, the stains seem to disappear, or become very
>Any ideas as to why this is happening, and what I can do about it.
>Research Project Coordinator
>Neuroscience and Mental Health
>Hospital for Sick Children
>Mc Master Building, RM 3006
Microscopy Facility Supervisor
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
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