Re: [Histonet] HRP Substrates

From:Constance McManus

I'm using NOVA Red and it works great.  It seems to be about like AEC -- has a rich, deep red color  -- except that it goes through alcohols and histoclear nicely. The data sheet states that it needs to be prepared in glass distilled water, which I do.  I don't know what would happen if you used regular DI, I imagine it would form a precipitate or not be as rich a red color.  It also needs to be prepared within a couple of minutes before using.  But it's not difficult to use and I highly recommend it. 

Connie McManus, HT
University of Utah 
School of Medicine, 
Dept. of Dermatology

>What about NOVA Red from Vector?  It withstands alcohols, but how much I am 
>not certain of.  You can contact Craig Pow at VECTOR  email 

>He is tech services and very helpful

At 10:28 AM 6/26/2007, you wrote:
>Hi all. I am looking for a peroxidase substrate that will withstand
>embedding. Something other that DAB. AEC and TMB are not stable thru
>embedding if memory serves me right. Any suggestions?

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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