Re: [Histonet] Eosin on processor

From:Dawn Cowie



We also do this. It does not interfere at all with the H&E stain or subsequent IHC's. Yes, it is a life saver for small bx's. It makes it much easier to find them in the cassette when embedding and also when they are being cut - they show up very nicely in the block. You can see more easily if you are going deep enough for a good section, etc.
   
  DLC

Joe Nocito  wrote:
  Linda,
my last lab put 25 mls of Eosin in the first 100% alcohol container on each 
of the tissue processors. It didn't interfere with the H&E staining, it did 
stain up the tissue processors, but wasn't a factor as to their operations. 
Since the lab processed mostly biopsies, the Eosin was a life saver, 
especially when it came to GI and prostate biopsies.
Hope this helps.

Joe
----- Original Message ----- 
From: "Linda Hines" 

To: 
Sent: Thursday, June 28, 2007 5:48 PM
Subject: [Histonet] Eosin on processor


>I would like to know which of the labs are using Eosin on their tissue 
>processor to mark the small specimens. Also if you have a procedure, if it 
>has interfered with your processing, and the staining of your H&E. I've 
>seen this before and liked it when working with the finite, and some of the 
>needle bx's. This is just one of my sudgestions for our lab. Thanx for 
>your input.
> Linda
> TRMC
>
>
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