The pH can be critical for cresyl violet to work--check your protocol to see
what pH is recommended for this application. Also, the stain will
gradually be depleted from the solution after long use, so sometimes making
up a fresh batch can improve your results. Good luck! Susan
----- Original Message -----
From: "Benjamin Cottam"
To:
Sent: Monday, June 25, 2007 5:46 PM
Subject: [Histonet] Cresyl Violet Staining
>I have been staining rodent and moonkey retina fixed frozen sections
> with cresyl violet, .5% for some time. Recently the staining has been
> washing out in the rinsing/dehyration steps. I have tried inceasing the
> time in the stain, increasing the stain concentration to 1%, and even
> shortening the time in the wash and dehration. I also tried moving
> right from CV to 95% EtOH but after a few minutes, the staining has
> washed out of the retina. Any suggestions on what to do next would be
> most helpful.
> Benjamin Cottam
> Sr. Research Assistant
> OHSU-CEI
>
>
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