Re: [Histonet] Cresyl Violet Staining

From:"Susan Bachus"

The pH can be critical for cresyl violet to work--check your protocol to see 
what pH is recommended for this application.    Also, the stain will 
gradually be depleted from the solution after long use, so sometimes making 
up a fresh batch can improve your results.   Good luck!    Susan
----- Original Message ----- 
From: "Benjamin Cottam" 
Sent: Monday, June 25, 2007 5:46 PM
Subject: [Histonet] Cresyl Violet Staining

>I have been staining rodent and moonkey retina fixed frozen sections
> with cresyl violet, .5% for some time.  Recently the staining has been
> washing out in the rinsing/dehyration steps.  I have tried inceasing the
> time in the stain, increasing the stain concentration to 1%, and even
> shortening the time in the wash and dehration.  I also tried moving
> right from CV to 95% EtOH but after a few minutes, the staining has
> washed out of the retina.  Any suggestions on what to do next would be
> most helpful.
> Benjamin Cottam
> Sr. Research Assistant
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