RE: [Histonet] teeth

From:"Rittman, Barry R"



Roberta
I have to disagree with both of the suggestions that you have received
concerning the processing of teeth.
In my hands both these methods produced far inferior results to the use
of either Kristensen's Sodium Formate/Formic acid or of disodium EDTA
for demineralization of teeth.
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet
Maass
Sent: Monday, June 18, 2007 8:58 PM
To: Roberta Horner
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] teeth

Roberta

I would recommend 10% trifluoroacetic acid for teeth.  The procedure is 
printed in "The Journal of Histotechnology/ Vol. 25, No. 3, September
2002. 
Article titled "Processing the Complete Canine Tooth".

Janet Maass


----- Original Message ----- 
From: "Roberta Horner" 
To: 
Sent: Monday, June 18, 2007 1:23 PM
Subject: [Histonet] teeth


I just received some teeth to section can anyone tell me if I can just
use any decalcification solution or is there something special to do
with teeth?  These are from a calf and it has been over 20 years since I
took class for histology and in the back of my mind I seem to remember
that you need to do something different.

Roberta Horner HT, HTL

Penn State University

Animal Diagnostic Lab

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