RE: [Histonet] de Galantha stain for Gout

From:"Kellar, Eric C"

De Galantha's Method for Urate Crystals

Fixation:	Absolute alcohol (4 hours for 2mm thick specimens).
Paraffin:	From absolute alcohol transfer blocks to xylene-paraffin, equal parts, at 58C for 2 hours. Place in paraffin for 1 hour. Embed.
Microtomy:	Cut sections at 8 microns.

Developing solution:

3% Gelatin in hot water		10 ml
10% Silver Nitrate		  3 ml
2% Hydroquinone		  2 ml
Prepare just before use.

1. Place slides in xylene, then absolute alcohol, 2 changes each. Avoid water.
2. Place in 10% silver nitrate solution. Expose to strong sunlight until urates are a bright rose; approximately 3 hours.
3. Prepare developing solution and pour over slides until the urates turn black and connective tissue is yellow.
4. Wash slides quickly in hot water 58C.
5. Dehydrate slides in absolute alcohol, three changes, clear and mount.

Results:		Urates			Black
		Background		Yellow

Reference:	Histopathological Methods and Color Atlas of Special Stains and Tissue Artifacts, Lee Luna 1992.	
Eric C. Kellar
Quest Diagnostics 
Histology Laboratory Supervisor
South Florida

-----Original Message-----
From: []On Behalf Of Weems, Joyce
Sent: Monday, June 25, 2007 12:31 PM
To: Histonet
Subject: [Histonet] de Galantha stain for Gout

Anyone have this procedure? I can only find references - not the actual procedure.

Need today if possible. Thanks! j

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

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