De Galantha's Method for Urate Crystals
Fixation: Absolute alcohol (4 hours for 2mm thick specimens).
Paraffin: From absolute alcohol transfer blocks to xylene-paraffin, equal parts, at 58C for 2 hours. Place in paraffin for 1 hour. Embed.
Microtomy: Cut sections at 8 microns.
3% Gelatin in hot water 10 ml
10% Silver Nitrate 3 ml
2% Hydroquinone 2 ml
Prepare just before use.
1. Place slides in xylene, then absolute alcohol, 2 changes each. Avoid water.
2. Place in 10% silver nitrate solution. Expose to strong sunlight until urates are a bright rose; approximately 3 hours.
3. Prepare developing solution and pour over slides until the urates turn black and connective tissue is yellow.
4. Wash slides quickly in hot water 58C.
5. Dehydrate slides in absolute alcohol, three changes, clear and mount.
Results: Urates Black
Reference: Histopathological Methods and Color Atlas of Special Stains and Tissue Artifacts, Lee Luna 1992.
Eric C. Kellar
Histology Laboratory Supervisor
From: firstname.lastname@example.org [mailto:email@example.com]On Behalf Of Weems, Joyce
Sent: Monday, June 25, 2007 12:31 PM
Subject: [Histonet] de Galantha stain for Gout
Anyone have this procedure? I can only find references - not the actual procedure.
Need today if possible. Thanks! j
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