RE: [Histonet] SISH - Ventana

From:"Bowlay, Greg"

Hi Gudrun,
We have been trialling HER2/Ch17 SISH on a Ventana Benchmark and it works very well!! We are very impressed by the contrast between signal and counterstain, particularly in the polysomy amd low amp cases. Another advantage is that the tissue architecture is maintained greatly, and also inclusion of control tissue on same slide. At around six hours for the complete run it increases our TAT's and volume.
We are looking at Extended CC2 and four minutes ISH protease 3 for our tissue - which preserves tissue integrity compared to the technique we currently use for CISH (Zymed).
We recommend it highly!!!
Greg Bowlay
Anatomical Pathology
Mater Health Services
South Brisbane Q 4101
PH: +61 7 3840 8586
FAX: +61 7 3840 8338
"Far better it is to dare mighty things to win glorious triumphs even though chequered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the grey twilight that knows neither victory nor defeat." Theodore Roosevelt


From: on behalf of Gudrun Lang
Sent: Fri 29/6/07 1:40 AM
Subject: [Histonet] SISH - Ventana

I would be interested in people's experience with the SISH-test on the

SISH = Silver in situ hybridisierung for detecting Her2neu-gene copies.

The principle is metallography, where the bound HRP catalyses the reduction
of Silveracetat. This results in black dots, that can be detected with a
light microscope.

Thanks in advance

Gudrun Lang

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