Flame it with a bunsen.

What?

Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
Pathology Services Manager
Weston General Hospital
North Somerset

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Asf2k3@aol.com
Sent: 01 July 2007 00:37
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40


What is the best way to avoid having bubbles in mold when embedding
tissue. 
We just use regular melted parrafin in embedding center. What I did is
put 
parraffin in mold, then put in on cold plate to start freezing a bit,
then  put 
the tissue so it will adhere to mold, then put the cassette top on mold,
and  
fill it up with parrafin. I do this step but still see bubbles forming.
I tap  
the cassette top on mold to get rid of bubbles which works, but this
step is 
not  convenience if you have to embed quickly because I have to embed
like 120 
blocks  in hr and half.
 
A
 
Please let me know. 



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