RE: [Histonet] Nuclear artefact

From:"Marshall Terry Dr, Consultant Histopathologist"

Look more closely. They are not nuclear.


-----Original Message-----
[] On Behalf Of Gale,
Sent: 27 June 2007 17:54
Subject: [Histonet] Nuclear artefact

Urgent help required!!!
I know that I've seen previous messages regarding this artefact but need
some current views on the subject.
I am seeing nuclear artefact in some of our formalin fixed paraffin
embedded tissues.  The artefact appears as vacuoles or bubbles in the
nuclei with H&E.  I have tried multiple things to fix this to no avail.
Please, please provide your wisdom as to what could be causing this.  I
am at a loss!!!
Many thanks in advance,
Nadia Gale
Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th
Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 _______________________________________________
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