RE: [Histonet] Formaldehyde: tissue degradation

From:"Rittman, Barry R"

Krish Soni
I am not sure precisely the information that you are looking for.

There are a plethora of factors that are involved here. 
For example -are you concerned with loss of microanatomy, of a specific
tissue or of components such as amino acids or proteins compared to
lipids, compared to carbohydrates?
Are you trying to determine the loss of staining or of histochemical or
immunochemical reactions?
If you could clarify this it would be much appreciated.

Original Message-----
[] On Behalf Of Geoff
Sent: Tuesday, June 19, 2007 9:45 AM
Subject: Re: [Histonet] Formaldehyde: tissue degradation

I would start with the classic texts:

*The Microtomist's Vade-Mecum.* Bolles Lee, Arthur. 10th edition, 1937. 
J.B. Gatenby and T.S. Painter, editors. P. Blakiston's Son and Co., Inc.

*The Microtomist's Formulary and Guide.* Gray, Peter; 1954. The 
Blakiston Co., Inc.

*Selected Histochemical and Histopathological Methods.* Thompson, Samuel

W. 1966, Charles C. Thomas.

*Histological Techniques.* M. Gabe (English edition, transl. E. Blackith

and A. Kavoor). Masson, Paris, 1976

Also, how are you going to define "valid scientific evaluation"?


Pankaj Krish Soni wrote:
> Dear All
> We are trying to identify some research that studies the degradation
> tissue kept in formaldehyde over long periods of time (over 5 years).
> Ultimately we are trying to assess the number of years beyond which
> quality of tissue fixed in formaldehyde would not generally permit
> scientific evaluation.
> Does anyone know of any relevant research?
> Any help would be very much appreciated.  Thank you.
> Krish Soni
> _______________________________________________
> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

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