Thanks to all who reponded to my query regarding excessive background. I
still believe that most of the problem is to do with the decalcification of
the tissue, as I have got beaytiful clean & specific staining in controlled
tissues with the sam Abs and Tris/Tween wash buffer.
Well, we do our best with what we got...and try to get the researchers to
understand that you gotta plan ahead!
Bone Research Unit
University of the Witwatersrand
"There are nights when the wolves are silent and only the moon howls".
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