[Histonet] picro sirius red

From:"Liz Chlipala"

Hello everyone
We are running some picro sirius red for image analysis and were running into a bit of a problem.  We are staining rat heart, the collagen staining is quite nice but some of the muscle is staining a tad bit orangish rather than a really nice yellow.  Which is not a problem when you look under the microscope but once the images are captured for analysis thats were we run into problems.   We have tried making up fresh staining solution, etc.  differenciating more in the acetic acid but the muscle is stil a bit orange in color.  Any help would be appreciated.  
Thanks in advance
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com
HYPERLINK "http://www.premierlab.com/"www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

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