[Histonet] permeabilize the embryos-Immunohistochemistry with whole mouse embryos

From:sohail ejaz

Dear Friends

I am trying to stain whole mount of embryo E9.5 and E10.5 using undermentioned way, but i am unable to get any positive stainning to embryonic vasculature which might be a problem of permiabilization of embryo.
  So i would like to enquire about some chemicals and their exact concentrations which can be helpful to solve this issue.
  I am using follwoing pattern
  5% H2O2 in methanol for 5 hrs
  (Primary antibody) Rat antimouse CD31, Cat log No. 550274  1/50
  (2ndry antibody) HRP conjugated affinipure goat anti Rat IgG code 112-035-167, 1/100 
  Jakson Immuno Research lab.
  Vector DAM, Sk 4100, Vector lab

Fussy? Opinionated? Impossible to please? Perfect.  Join Yahoo!'s user panel and lay it on us.
Histonet mailing list

<< Previous Message | Next Message >>