Hi all, 
I was wondering for those of you out there familiar with staining for
Retinoblastoma (Rb) defects what products do you use and how clean are
the results? I tend to get variable cytoplasmic staining (it's supposed
to be nuclear only) in the various tissue microarrays we're looking at.
[lower ab  [] also weakens nuclear expression]  This may be perfectly
normal, but all the published images show only the very sharp, clean
nuclear staining. I also do not frequently see the "internal positive
control" staining of endothelial cells. 
TIA, your input is always appreciated, 
Melissa

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