I am carrying out c-fos staining on brain tissue, and have run into a bit
of a problem. The staining seems to work fine up untill I mount the sample
with a xylene based mountant (permount). The stains are there after alcohol
washing, but once I mount it, the stains seem to disappear, or become very
very faint.

Any ideas as to why this is happening, and what I can do about it.

Thanks,

Jaina Negandhi
Research Project Coordinator
Neuroscience and Mental Health
Hospital for Sick Children
Mc Master Building, RM 3006
Tel: 416-813-6551
Fax: 416-813-8724


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