[Histonet] Re: Nuclear artefactI


Nadia Gale, Histotechnologist at the Centre for Translational and Applied Genomics (CTAG)
in Vancouver, BC asks:

>>I am seeing nuclear artefact in some of our formalin fixed paraffin
embedded tissues. The artefact appears as vacuoles or bubbles in the
nuclei with H&E. I have tried multiple things to fix this to no avail.<<
I think we've discussed this topic before on Histonet, and maybe I just
wasn't paying attention - but I would really like to know how to trouble
shoot this common artifact: just what do you do first, and next, and after

In my travels as a locum tenens pathologist this is one of the most common
problems I see in laboratories. Always everybody is in denial that any
such thing exists. In particular, this bubble artifact makes it impossible
to assess the nuclear grade of breast cancers and other tumors requiring
nuclear grading.

Bob Richmond
Samurai Pathologist
Knoxville TN


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