What is the best way to avoid having bubbles in mold when embedding tissue.
We just use regular melted parrafin in embedding center. What I did is put
parraffin in mold, then put in on cold plate to start freezing a bit, then put
the tissue so it will adhere to mold, then put the cassette top on mold, and
fill it up with parrafin. I do this step but still see bubbles forming. I tap
the cassette top on mold to get rid of bubbles which works, but this step is
not convenience if you have to embed quickly because I have to embed like 120
blocks in hr and half.
A
Please let me know.
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