What do you do when you have a polyclonal antibody for which the vendor
has not determined an Ig concentration? It's easy to match
concentration and isotype for a monoclonal but can be impossible for a
polyclonal. If we have an Ig concentration, we will match with Rabbit
IgG. If not, do you count on a buffer control, irrelevant antibody (not
concentration matched), or normal sera? Lots of choices, not sure what
is the best for this situation.
Tim Coskran
Pfizer
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