I have been staining rodent and moonkey retina fixed frozen sections
with cresyl violet, .5% for some time. Recently the staining has been
washing out in the rinsing/dehyration steps. I have tried inceasing the
time in the stain, increasing the stain concentration to 1%, and even
shortening the time in the wash and dehration. I also tried moving
right from CV to 95% EtOH but after a few minutes, the staining has
washed out of the retina. Any suggestions on what to do next would be
most helpful.
Benjamin Cottam
Sr. Research Assistant
OHSU-CEI
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