A PUBMED search led to a wonderful publication titled An evaluation of the
immunohistochemistry benefits of boric acid antigen retreival on rat
decalcified joint. Wilson, Elaine et al. J Immunological Methods V. 322,
Issue 1-2, Pages 137-142, 2007.
This had excellent information on fixation, low temperature retrievals, and
also decalcification of bone to see CD31 and PCNA
immunohistochemistry. Since CD31 is in Histonet inquiries so much
recently, it may be a help to those trying to stain for this marker.
If anyone needs the pdf, I will send privately.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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