While we don't flash freeze our embryos, we do fix in para and sink in
30% sucrose like you do. Then, instead of using just OCT to embed, we
use a 1:1 mix of OCT and 30% sucrose. This helps when sectioning, if
that seems to be the problem. I'm not sure if this solution will work
with fast freezing, we freeze our embedded embryos on dry ice.
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