Re: [Histonet] Perfusing Rat Lung

From:Geoff McAuliffe

Hi alexina:

First off, why replace the fixative with Ringer's? Remember formalin 
works slowly so removing it will work against good morphology.
What about the morphology is not to your liking?
If you instill fixative down the trachea with the lungs in the animal 
and the chest cavity closed you might get better results.


Alexina Wempren wrote:
> Does anyone have a good protocol for perfusing rat lung?  We have been
> flushing with ringers, then 10% NBF, then ringers and ending with 30%
> sucrose overnight but the morphology is not good. We add 0.8cc of air so
> the airways and vessels are not flat but it's still not great. We used
> to use paraformaldehyde but my supervisor did not like it. Any help
> would be greatly appreciated.
>    Thanks,           alexina
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