Re: [Histonet] Perfusing Rat Lung

From:"Greg Dobbin"

I have 2 references for you:

Microscopic changes induced by the intratracheal inoculation of amniotic fluid and meconium in the lung of neonatal rats. J. Martinez-Burnes, et al.  Histology and Histopathology (2002) 17:1067-76.


Ultrastructural changes in the lungs of neonatal rats intratracheally inoculated with meconium. J. Martinez et al.  Histology and Histopathology (2003) 18:1081-94.

If you prefer, I can fax you the Materials and Methods section(s) that describe the perfusion protocol that produced amazing results!

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

>>> "Alexina Wempren"  6/5/2007 2:29 PM >>>
Does anyone have a good protocol for perfusing rat lung?  We have been
flushing with ringers, then 10% NBF, then ringers and ending with 30%
sucrose overnight but the morphology is not good. We add 0.8cc of air so
the airways and vessels are not flat but it's still not great. We used
to use paraformaldehyde but my supervisor did not like it. Any help
would be greatly appreciated.

   Thanks,           alexina

Histonet mailing list

Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization.  If you have received this communication in error, please notify the sender immediately.  If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.  

Déclaration de confidentialité
Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique.

Histonet mailing list

<< Previous Message | Next Message >>