Re: [Histonet] Frozen sectioning of mouse mammary tissue

From:Derek Papalegis

Drop the temp down to -30. That usually helps. Spray the blade and the 
anti-roll plate with cryo spray as well.

-Derek wrote:
> Hi all,
>    I have a grad student who needs help with frozen sectioning of mouse mammary
> tissue. He has not been able to cut any sections because it is fatty tissue. I
> haven't been able to watch him cut yet; I will be doing that tomorrow. In the
> meantime, if someone has suggestions and also a protocol, that would be very
> helpful. Thanks again.
> Meg Berger
> Histologist
> Animal and Poultry Sciences
> VA Tech
> _______________________________________________
> Histonet mailing list

Derek Papalegis HT (ASCP)
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354

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