Re: [Histonet] Fat dissolving reagent

From:Rene J Buesa

  I think that you are referring to the "lymph node revealing solution", with the follwoing recipe:
  100% ethanol ----------2.0 L
  distilled water ----------0.68 L
  40% formalin------------0.320 mL
  acetic acid --------------0.2 L
  René J.

"Hannen, Valerie"  wrote:
  Hi Patricia,

We do not use a " fat dissolving" solution to find our lymph nodes.
However, we do use a great product on colonic and axillary adipose
tissues, that greatly helps in finding the nodes. It is called "O-FIX",
and we purchase it from Surgipath.
If time in solution is not extremely critical...we leave the adipose
tissue in the solution overnight at room temperature.
If time is critical, we place the tissue in the solution and heat it to
@ 43 degrees centigrade for approximately 3 hours with the lid loosely
on the container (otherwise pressure builds up and the top can "blow"

Hope this helps.

Valerie Hannen, MLT(ASCP),HTL,SU(FL)
Parrish Medical Center
Titusville, Florida

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