A workshop director years ago suggested using a reticulin stain in
conjunction with a Massons trichrome and the routine H&E stain. They did
not counterstain the reticulin stain although an eosin stain would be nice
OR nuclear fast red for red/black contrast.
You should see correlation between reticulin stain and Masson's
trichrome. Remember that Mass Tri stains ALL types of connective tissues
that include basement membrane, reticulin and collagen. You could, for
fun, do a Verhoeffs van Giesons for just collagen fibers too - you will
have an interesting collection of stains in the end.
Try to pick up adjacent sections in order to have a closer correlation
within 25 um or so of each section
Pick up and stain in this order (or what you prefer)
2. Mass Tri
3. Verhoeffs van Gieson
Then repeat the sequence.
At 09:08 AM 6/8/2007, you wrote:
>Dear Histonet Community-
> Currently I am working on a capstone project dealing with Cirrhosis of
> the liver. If anyone has experienced working with Cirrhosis, please
> contact me, I have some questions. And anything that anyone could offer
> me would be great. Right now I am using the Masson Trichrome as a special
> stain for this disease, any other suggestions that could possibly be a
> better stain, please let me know. Thank you
> Robyn Pulsifer
> Histology Lab Assistant
> Argosy University
> Eagan, MN
>Building a website is a piece of cake.
>Yahoo! Small Business gives you all the tools to get online.
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Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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