RE: [Histonet] Question about Coverslippers & Barcoded Slides

From:"Kellar, Eric C"



Charlotte,

I have success with the Sakura Tissue-Tek film coverslipper set on "short" coverslip with the Ventana bar code labels. The glue does deposit on the chain drive, but daily cleaning will avoid, 'gunking up the works'.  You may be able to purchase a used one. It's better than coverslipping 300+ slides by hand or standing guard with your finger on "STOP" button over the glass coverslipper. Reduction of the clearing agent levels prior to coverslipping helps too.

Eric C. Kellar
Quest Diagnostics 
Histology Laboratory Supervisor
South Florida


 -----Original Message-----
From: 	histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]  On Behalf Of Kopczynski, Charlotte
Sent:	Monday, June 11, 2007 1:19 PM
To:	histonet@lists.utsouthwestern.edu
Subject:	[Histonet] Question about Coverslippers & Barcoded Slides

Can anyone out there give me some feedback on auto-coverslippers and
Ventana bar-coded slides.  We have found that our Leica coverslipper
will not work on slides that have a barcode label (example Ventana
Immuno stained slides)  We thought that the TBS coverslipper would work
and did on dry slides with barcode labels.  However, after purchasing a
TBS coverslipper, we found that after the slides had been dehydrated and
cleared in xylene, there is some glue from the barcode label which oozes
out the sides of the label and the slides get stuck in the coverslipper.
Has anyone else found a coverslipper that works or any recommendations.
We are immunostaining 300+ slides per day and manual coverslipping is a
problem.

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Regional Pathology Manager
BayCare Laboratories
Phone: 727-461-8246
Fax:   727-462-7596
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