It's been a few years since I did research, but we used to just use
10% NBF and we never had any complaints...you might want to give it a
CHristine Tambasco, HT (ASCP)
St. Marys Hospital @ Amsterdam, New York
From: "Alexina Wempren"
Subject: [Histonet] Perfusing Rat Lung
Date: Tue, 5 Jun 2007 10:29:32 -0700
>Does anyone have a good protocol for perfusing rat lung? We have
>flushing with ringers, then 10% NBF, then ringers and ending with
>sucrose overnight but the morphology is not good. We add 0.8cc of
>the airways and vessels are not flat but it's still not great. We
>to use paraformaldehyde but my supervisor did not like it. Any
>would be greatly appreciated.
> Thanks, alexina
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