RE: [Histonet] P53 survey

From:"Sebree Linda A."


We use Biocare's p53 at 1:50 with Biocare's Bull's Eye, Diva or BORG for
2" in their Decloaking chamber (pressure cooker).  Or we use Ventana's
standard CC1 HIER on our VMS BenchMark and XT.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
[] On Behalf Of James L
Sent: Monday, June 11, 2007 12:41 PM
Subject: [Histonet] P53 survey

Dear Histonetter's

I'm curious, what p53 clone do you use and what is your preferred HIER 
pretreatment on FFPE human tissue?


Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"
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