Jim,

We use Biocare's p53 at 1:50 with Biocare's Bull's Eye, Diva or BORG for
2" in their Decloaking chamber (pressure cooker).  Or we use Ventana's
standard CC1 HIER on our VMS BenchMark and XT.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James L
Burchette
Sent: Monday, June 11, 2007 12:41 PM
To: histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: [Histonet] P53 survey


Dear Histonetter's

I'm curious, what p53 clone do you use and what is your preferred HIER 
pretreatment on FFPE human tissue?

Sincerely,

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"
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