Hello Jim
I use Dako's p53 clone DO-7 as well with Dako's Target Retrieval
Solutin in a steamer.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Dawson, Glen"  06/11/07 2:06 PM
>>>
Jim,

I use Dako's monoclonal p53 (clone DO-7) with Dako's pH 6.0 citrate
target retrieval for my HIER.  It works very well.

Good Luck,
Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of James L
Burchette
Sent: Monday, June 11, 2007 12:41 PM
To: histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu 
Subject: [Histonet] P53 survey


Dear Histonetter's

I'm curious, what p53 clone do you use and what is your preferred HIER

pretreatment on FFPE human tissue?

Sincerely,

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"
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