RE: [Histonet] Laser capture microscopy to isolate sebocytes

From:"Sherwood, Margaret "


I referred you to Molecular Devices (Arcturus Division).  I'm not sure what LCM
instrument you are using, but they sell the kits you are interested in. =20


Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 

-----Original Message-----
[]On Behalf Of
Castillos, Luminita
Sent: Wednesday, June 06, 2007 10:42 AM
Subject: [Histonet] Laser capture microscopy to isolate sebocytes

Hi everybody, 


Does somebody have experience on laser capture microscopy to isolate
human sebocytes? I have some skin punch biopsies and I need to perform
LCM to isolate sebocytes for RNA purification, amplification and gene
expression analysis. I am worried about: what type of staining I should
perform? What kind of RNA extraction kit I should use for these
particular cells and what is the RNA yield I should expect? I deeply
appreciate any feedback for this particular issue. Thank you in advance.



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