Liquid Nitrogen helps too! The spray kind, not emersion. The OCT gets too cold and cracks before the tissue gets cold enough.
From: firstname.lastname@example.org on behalf of Derek Papalegis
Sent: Tue 6/5/2007 11:30 AM
Subject: Re: [Histonet] Frozen sectioning of mouse mammary tissue
Drop the temp down to -30. That usually helps. Spray the blade and the
anti-roll plate with cryo spray as well.
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