RE: [Histonet] Cyclin D1 antibody from Cell Marque user experiences

From:"Bowlay, Greg"

Hi Sarah,
We use Cyclin D1 SP4 from Neomarkers, like young Barry, but we use a dilution of 1:200 on Ventana Benchmark requiring only mild CC1 (on-board retrieval), primary incubn of 32 minutes using the iView detection system.
Greg Bowlay
Anatomical Pathology
Mater Health Services
South Brisbane Q 4101
PH: +61 7 3840 8586
FAX: +61 7 3840 8338
"Far better it is to dare mighty things to win glorious triumphs even though chequered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the grey twilight that knows neither victory nor defeat." Theodore Roosevelt


From: on behalf of Sarah O'Bryan
Sent: Mon 11/6/07 4:14 PM
Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences

Hello all,

I would like some information or feedback on how people find different
Cyclin D1 antibodies? I can see from some previous messages that it can
be a difficult antibody to optimize... I am particularly interested in
Cell Marque's users, as we have a customer who would like to know of
user experiences with this antibody before she decides whether to trial
or not.

If you use Cyclin D1 in your laboratory, would you please let me know
what clone you use, who manufactures it, and what you think of it? What
is the leading antibody on the market?

Thanks for your help.


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