RE: [Histonet] Cyclin D1 antibody from Cell Marque user experiences

From:"Barry Madigan"

Hi Sarah,

We use Cyclin D1 Clone SP4 from Lab Vision at a dilution of 1:50 using high
pH heat retrieval.
This is on a BondMax immunostainer with the Polymer Refine detection system.
Excellent results.

Barry Madigan
Royal Brisbane Hospital

-----Original Message-----
[] On Behalf Of Sarah
Sent: Monday, 11 June 2007 4:15 PM
Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences


Hello all,


I would like some information or feedback on how people find different
Cyclin D1 antibodies? I can see from some previous messages that it can
be a difficult antibody to optimize... I am particularly interested in
Cell Marque's users, as we have a customer who would like to know of
user experiences with this antibody before she decides whether to trial
or not.


If you use Cyclin D1 in your laboratory, would you please let me know
what clone you use, who manufactures it, and what you think of it? What
is the leading antibody on the market?


Thanks for your help.





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