Thanks you to all the people who responded. A summary of the suggestions
I received follow:
* Following bluing-use 1 minute water wash followed by 70% alcohol
then place in Eosin
* No 70% alcohol after Eosin, 95% is dilute enough
* Use fresher tissues
* Add phloxine to the Eosin
* Treat slides with permanganate and oxalic acid before staining
* Check whether Eosin is water or alcohol soluble
Janet Keeping A.R.T., B. Voc. Ed
Instructor
Medical Laboratory Sciences
1 Prince Philip drive
P.O. Box 1693, St. John's
NL, Canada A1C 5P7
709 758 7657 tel
709 758 7635 fax
E-mail; janet.keeping@cna.nl.ca
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