[Histonet] Poor Eosin staining after prolonged fixation

From:"Keeping, Janet"

Has anyone experienced poor eosin counterstaining using tissues which
have had very prolonged fixation in neutral buffered formalin? The
tissues I have for students have been fixed for a very long time. (not
generally a problem in the clinical site). Although the pH of the stain
is between 4.6-5.0, the slides are well washed following bluing, time in
70% alcohol for differentiation is minimal  and eosin staining times
have been increased the counterstaining is still poor. I am guessing
that the tissue groups that bind with eosin are not accessible due to
continued cross linking. Any ideas or suggestions? 


Janet Keeping A.R.T., B. Voc. Ed


Medical Laboratory Sciences


1 Prince Philip drive

P.O. Box 1693, St. John's 

NL, Canada A1C 5P7

709 758 7657 tel

709 758 7635 fax

E-mail; janet.keeping@cna.nl.ca


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