.....been a lot of talk about this already, I'm aware of that. Now I
want to know if there is any "real" scientific" basis for the new CAP
guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no
more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed,
My sense is that this guideline really does represent best practice.
Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!!
However, is there really some difference in performance of the Her-2 IHC
assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or
72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to
be so much more of issue than OVER-fixation.
It's this top end that bothers me so much!!!
I am not aware of real data to support this new guideline! Does anyone
out there know anything different?????
Robert L. Lott, HTL(ASCP)
Manager, Anatomic Pathology
LabFirst / Trinity Medical Center - formerly
Montclair Baptist Medical Center
800 Montclair Road
Birmingham, AL 35213
Histonet mailing list