Re: [Histonet] threshhold for antibody binding

From:Rene J Buesa

  I had never experienced such a shart "stain-to-not at all" gradient. When diluted below the optimal ratio what I always found was weak staining, but not "no bind at all".
  I do not know why this could happen.
  René J.

"Smith, Allen"  wrote:
  We have an antibody that binds well at a 1:75 dilution, but does not appear
to bind at all when the dilution is 1:85. Has anyone noticed a similar
phenomenon: a sharp dilution threshhold below which the antibody works well
and above which it does not work at all?

Allen A. Smith, Ph.D.

Professor of Anatomy

Barry University School of Graduate Medical Sciences

Podiatric Medicine and Surgery

Miami Shores, Florida 33161

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