Re: [Histonet] histoclear vs xylene

From:Rene J Buesa

  In a "nutshell" I can tell you the following:
  1-xylene: universally used for tissue processing and staining but a product that should be avoided because of known health hazards.
  2-histoclear: a "xylene substitute" sometimes used as a replacement of xylene. Does not work equally well. It is "allegedly safe" but really with no documentation of long term health effects as xylene has. Maybe with time it will turn out to be as hazardous as xylene.
  Our profession has some health hazards and we just have to work as safely as possible.
  Hope this will help you!

"Campbell, John Thomas (UMR-Student)"  wrote:
  I have read several protocols on staining slides and most of them talk about using xylene the protocol that I am currently using says to use Histoclear. I was wondering what the difference was and I would like to know the pros and cons on using xylene and using histoclear. what do you all use and what exactly are the hazards of using xylene

John Campbell
Graduate Student 
University of Missouri Rolla
Lab phone 573-341-4069
Cell phone 573-694-0596

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