Re: [Histonet] Prussian Blue staining-thick brain sections

From:Geoff McAuliffe

Hi Sarah:

    Look up Stain Technology 50(2):87-91, 1975. I don't know if this 
would work for plastinated tissue. The references might be useful.

Geoff (UC 1978, when the Anatomy dept.was Anatomy)

Pixley, Sarah (pixleysk) wrote:

>Dear Histonet:
>Second message from me today, but different subject. I am staining
>sections (approx. 1 cm thick) of human brains, taken from the cadavers
>that were used by the first year medical students. I am staining them
>with Prussian Blue for use in the medical student neuroanatomy teaching
>labs. I am thinking of trying to do some fancier staining and our own,
>in house, plastination of the sections after staining. However, there
>are lots of methodological questions that come up, both in the staining
>and in the plastination.  I have a fairly limited budget for this, hence
>the home plastination. If anyone has experience with this, would you be
>so kind as to email me separately so that we could have a discussion
>about this? 
>Sarah Pixley (
>Univ. Cincinnati
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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