Re: [Histonet] Marking specimens through decalcification

From:Gayle Callis

Do you mean an accession number for each nose?

We mark the nylon bag itself with soft lead pencil and also put a thick 
paper tag  with ID#  written in pencil inside the bag with the sample.  Be 
sure you close the bag with string, or things turned upside down get mixed 
up, we use the string to hang the bag in a huge container of 10% formic 
acid, and stir to get rid of bubbles (CO2, a results of 
decalcification).  Suspension aids in faster, even decalcification.

At 06:22 AM 6/23/2006, you wrote:
>Dear Histonet:
>I will be putting rodent nose tissues in 10% formic acid to decalcify
>(after perfusion fixation in 4% paraformaldehyde). I need to mark the
>tissues to keep track of them. What can I use that will withstand the
>several days in formic acid? Each nose will be in a separate Shandon
>biopsy bag.
>Sarah Pixley
>Univ. Cincinnati
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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