Re: [Histonet] IHC with p27?

From:"Andi Kappeler"

Hi Eva
We used to use mo-a-Kip1/p27, clone 57, at approx. 1 ug Ig/ml after HIER in 
pressure cooker, citrate. As this was several years ago, we did not test 
HIER with high pH buffers. Hope this helps.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Eva Andersson" 
Sent: Tuesday, June 20, 2006 3:31 PM
Subject: [Histonet] IHC with p27?

> Good morning,
> I want to do some IHC with a Kip1/p27 antibody from BD Transduction 
> laboratories (610241) but I can't find any references to a working 
> concentration. Has anybody used this antibody for IHC? What conditions did 
> you use?
> Thanks for all your help,
> Eva Andersson
> Research Assistant Georgetown University
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