Re: [Histonet] E.M. precipitate
What is the size and shape of the precipitate?
The usual suspects are uranium or lead crystals
or blobs from the staining, or could be a
phosphate deposit after osmium fixation. The
first things to check are: is the lead stain at
or above pH 12? If not, it will cause black
precipitate; do you use a phosphate buffer during
fixation? If yes, make sure it is washed out
before osmium fixation, or use a sodium-only
buffer (mono- and dibasice sodium, no potassium).
If that does't work, search the archives of the Microscopy Society of America:
There have been several discussions on this list about your problem.
>We are having problems with black precipitate on
>our E.M. photo's. Does any one mind sharing your
>procedure with me. It has been checked and it is
>not bacteria. We think it is lead?
> Fax (817) 250-5698
> Brent Hart
>Talk is cheap. Use Yahoo! Messenger to make
>PC-to-Phone calls. Great rates starting at
>Histonet mailing list
Microscopy Facility Supervisor
Department of Biology
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
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