RE: [Histonet] slicing paraffin embeded specimens

From:"Jasper, Thomas G."

Hey John,

What are you sectioning?  14 microns sounds awfully thick to me.  I am in the clinical lab world so maybe you are working on some kind of special project or something that requires thicker sections.  It is our standard here to cut at 4 microns or thinner.  Some folks may disagree with me on this point, but most people I know in the world of clinical histology section at 4 or 5 microns, or less depending on tissue types and applications.
One more suggestion for you John.  I would bet if you contacted the local hospital in your town, someone would be willing to give you a very cursory tour/demo of pathology/histology services.  I know these people are around, we are just a well hidden group of uniquely talented individuals.
Hope this helps.

Thomas Jasper
Anatomic Pathology Supervisor
SMDC Clinical Lab
Duluth, MN

-----Original Message-----
[]On Behalf Of Campbell,
John Thomas (UMR-Student)
Sent: Thursday, June 22, 2006 1:49 PM
To: histonet
Subject: [Histonet] slicing paraffin embeded specimens

I am just getting started in histology and I am having trouble keeping my slices from bunching up on the microtome. What can I do to keep this from happening? I am using a manual slicer, slicing the specimens into 14 microns thick slices.

John Campbell
Graduate Student 
University of Missouri Rolla
Lab phone 573-341-4069
Cell phone 573-694-0596

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